Your Name
Research Associate · Molecular Biology, Cell Culture, qPCR, ELISA
your.email@example.com | +91 XXXXX XXXXX | Hyderabad, India | linkedin.com/in/your-profile
Summary
Research associate with 2+ years in molecular biology and cell-based assay labs at biologics companies in Hyderabad and Bengaluru. Runs qPCR, ELISA, Western blot and protein purification under GLP, and has supported 3 biosimilar development projects with documented, reproducible data.
Experience
- Developed and validated qPCR assays for 12 target genes, tuning primer design and annealing temperatures to reach 95–102% efficiency and cut repeat runs by 60%.
- Established a His-tag protein purification workflow with Ni-NTA chromatography and SDS-PAGE checks, raising recombinant protein yield from 2 mg/L to 7 mg/L for ELISA development.
- Designed a sandwich ELISA for host-cell protein detection, achieving intra-assay CV below 8% across 300+ samples from 3 biosimilar development batches.
- Maintained GLP lab notebooks and instrument logs for 6 equipment items, passing 2 internal quality audits with zero major observations.
- Maintained 8 mammalian cell lines (HEK293, CHO, HeLa) under strict aseptic technique, with zero contamination events across 12 months of routine passaging and cryopreservation.
- Executed Western blots and SDS-PAGE for 150+ protein samples a week, cutting re-runs by 30% by standardising transfer and blocking conditions.
- Automated plate-reader data analysis with GraphPad Prism templates, reducing weekly assay reporting time from 6 hours to 2 hours for a team of 5.
Projects
- Tested 8 plant extracts against 25 clinical MDR E. coli isolates by broth microdilution, identifying 2 extracts with MIC below 64 µg/mL.
- Identified blaTEM, blaCTX-M and blaSHV resistance genes in 25 isolates by multiplex PCR, linking gene profiles to extract susceptibility in the final thesis.
Skills
Molecular Biology: PCR, qPCR, Cloning, DNA/RNA Isolation, Gel Electrophoresis
Protein & Immunoassays: ELISA, Western Blot, SDS-PAGE, Protein Purification, Ni-NTA Chromatography, Assay Development
Cell Culture: Mammalian Cell Culture, Aseptic Technique, Cryopreservation, Biosafety Cabinet Handling
Lab Practice & Data: GLP, Lab Documentation, Data Analysis, GraphPad Prism, MS Excel